Rougeulle group

X chromosome inactivation is critical for women development and linked to sex-specific susceptibilities to various diseases. To understand how XCI contributes to male-female differences, we model, in vitro, key steps of X-inactivation using, as an entry point, Human Embryonic Stem cells (hESC). hESC are reprogrammed to discover how XCI is initially established during the first days of embryonic development with a particular focus on the role of structural noncoding RNAs such as XIST and XACT. hESC are differentiated into specific cell types to assess the plasticity of XCI in distinct somatic tissues, including the hematopoietic compartment and organized tissue through the use of organoids.

Remarkable differences exist in the way X chromosome inactivation is set up between human and mouse in connection with drastically different network of regulatory lncRNA genes which our group contributed to decipher. We are now exploring phenotypic and mechanistic variability in X-inactivation across primates using non-human primate pluripotent stem cells to probe the degree of genetic and epigenetic innovation across a limited timescale, and the contribution of the noncoding genome to such evolution.

2023

• Rosspopoff O,  Cazottes E, Huret C, Loda A, Collier A, Casanova M, Rugg-Gunn P, Heard E, Ouimette JF, Rougeulle C. Species-specific regulation of XIST by the JPX/FTX orthologs . Nucleic Acids Research, 2023 Feb

2022

• Mahadik K, Rougeulle C. Study of X Chromosome Activity Status in Human Naive Pluripotent Stem Cells Using RNA-FISH Methods Mol Biol. 2022;2416:239-255

2021  

• Heard E, Rougeulle C. Digging into X chromosome inactivation. Science. 2021 Nov 19;374(6570):942-943.
• Moscatelli M., Rougeulle C. Dernières nouvelles du chromosome X: des principes généraux nuancés. Médecine/Sciences, 37 2 (2021) 152-158

 2020

• Ouimette JF, Rougeulle C. Many XCI-ting routes to reach the eXACT dose. Nat Cell Biol. 2020 Dec;22(12):1397-1398
• Cazottes E., Rougeulle C. Straight to the X: Modeling Human X Chromosome Inactivation in hESCs by FGF Signal Blockade.Cell Stem Cell. 2020 Sep 3;27(3):352-353
• Patrat C, Ouimette JF, Rougeulle C. X chromosome inactivation in human development. Development  2020 Jan 3;147(1).

2019

• Casanova M, Moscatelli M, Chauvière LÉ, Huret C, Samson J, Liyakat Ali TM, Rosspopoff O, Rougeulle C. A primate-specific retroviral enhancer wires the XACT lncRNA into the core pluripotency network in humans. Nat Commun. 2019 Dec 11;10(1):5652.

2018

• Furlan G, Gutierrez Hernandez N, Huret C, Galupa R, van Bemmel JG, Romito A, Heard E, Morey C, Rougeulle C.  The Ftx Noncoding Locus Controls X Chromosome Inactivation Independently of Its RNA Products. Mol Cell. 2018 Apr 20. pii: S1097-2765(18)30227-2.

2017

• Vallot C., Patrat C., Collier AJ., Huret C., Casanova M., Liyakat Ali TM., Tosolini M., Frydman N., Heard E., Rugg-Gunn P.J.and Rougeulle C. Competing action of XIST and XACT noncoding RNAs in the control of X chromosome activity during human early development. Cell Stem Cell. 2017 Jan 5;20(1):102-111.  

2016

• Vallot C.,  Ouimette JF., Rougeulle C. Establishment of X chromosome inactivation and epigenomic features of the inactive X depend on cellular contexts.  Bioessays. 2016 Sep;38(9):869-80​
• Ouimette JF, Rougeulle C. How Many Non-coding RNAs Does It Take to Compensate Male/Female Genetic Imbalance?  Adv Exp Med Biol. 2016;886:33-49

2015

• Vallot C*, Ouimette JF*, Makhlouf M, Féraud O, Pontis J, Côme J, Martinat C, Bennaceur-Griscelli A, Lalande M, Rougeulle C. Erosion of X Chromosome Inactivation in Human Pluripotent Cells Initiates with XACT Coating and Depends on a Specific Heterochromatin Landscape.Cell Stem Cell. 2015 May 7;16(5):533-46.

2014

• Makhlouf M.*, Ouimette JF.*, Oldfield A.*, Navarro P., Neuillet D. and Rougeulle C. A prominent and conserved role for YY1 in Xist transcriptional activation. Nat Commun. 2014 Sep 11;5:4878.

2013

• Häfner S. and Rougeulle C. La matière noire du génome. Pour la Science, 81: 58-63.
• Vallot C., Huret C., Lesecque Y., Resch A., Oudrhiri N., Bennaceur-Griscelli A., Duret L. and Rougeulle C. XACT, a long non-coding transcript coating the active X chromosome in human pluripotent cells. Nat Genet. 2013 Mar;45(3):239-41.​

2012

• Vallot C. and Rougeulle C. Epigenetic stability of human pluripotent stem cells. « Epigenomics: From Chromatin Biology to Therapeutics » 118-133.

2011

• Romito A. and Rougeulle C. Origin and evolution of the long non-coding genes in the X-inactivation center. Biochimie. 2011 Nov;93(11):1935-42.​
• Makhlouf M. and Rougeulle C. Linking X Chromosome Inactivation to Pluripotency: Necessity or Fate? Trends Mol Med. 2011 Jun;17(6):329-36.​

2010

• Navarro P., Oldfield A., Legoupi J., Festuccia N., Dubois A., Attia M., Schoorlemmer J., Rougeulle C., Chambers I. and Avner P. Molecular coupling of Tsix regulation and pluripotency. Nature 2010 Nov 18;468(7322):457-60.​
• Mitjavila-Garcia M., Bonnet M.L., Yates F., Haddad R., Oudrhiri N., Féraud O., Magniez A., Vallot C., Makhlouf M., Rougeulle C., Bennaceur-Griscelli A. and Turhan A.G. Partial reversal of the methylation pattern of X-linked gene HUMARA during hematopoietic differentiation of human embryonic stem cells. J Mol Cell Biol. 2010 Oct;2(5):291-8.​

2009

• Navarro P., Chantalat S., Foglio M., Chureau C., Vigneau S., Clerc P. Avner P. and Rougeulle C. A role for non-coding Tsix transcription in partitioning chromatin domains within the mouse X-inactivation centre. Epigenetics Chromatin. 2009 Jul 20;2(1):8.​

Claire ROUGEULLE, PhD, HDR, DR1 – CNRS
CNRS – Epigenetics and Cell Fate – UMR7216
Université Paris Cité
Bat Lamarck, 4ème étage. Case courrier 7042
35, rue Hélène BRION
75205 Paris Cedex 13

Tél : 33 (0)1 5727 8924
Fax : 33 (0)1 5727 8911
Email : claire.rougeulle@u-paris.fr