GENIE : Genome engineering in epigenetics

The “Genome ENgeneering In Epigenetics” (GENIE) platform focuses on the various applications of CRISPR/Cas technologies in the field of epigenetics.

GENIE assists researchers in the planning of experiments and the assembly of reagents for the generation of custom experimental systems. We provide expertise and tools for various genome and epigenome engineering applications.

Our goal is to facilitate the choice of genome editing, epigenome and other tools based on CRISPR/Cas technologies. GENIE is open to all members of the UMR7216 Epigenetics and Cell Fate unit and to a certain extent to members of the transverse programs of the Labex “Who am I?”. The acceptance of external projects is possible after discussion with the steering committee and depends on the resources available.

Expertise

Our expertise lies in the following areas:

• Genome editing: knock-out, knock-in, and point mutation introduction
• Modulation of gene expression and modification of the epigenome
• Use of CRISPR/Cas technologies for imaging or for identification of protein composition of the locus of interest using proximity tagging techniques
• CRISPR/Cas targeting of RNA for degradation, imaging, or editing