EPI2 Imaging Platform

Scientific manager:

Sophie Polo (DR1 Inserm)

Technical manager:

Sandra Piquet (IE CNRS)

High-throughput microscopy manager:

Audrey Chansard (IE CNRS)

Steering Committee:

• Dounia Djeghloul (DR2 CNRS)
• Pierre Therizols (CR CNRS)
• Jacob Seeler (CR Pasteur)

Plessier A, Chansard A, Petit E, Novion Ducassou J, Couté Y, Polo SE.
Proteomic profiling of UV damage repair patches uncovers histone chaperones with central functions in chromatin repair.
Nat Commun. 2026 Jan 28;17(1):2127 ; PMC12957454

Saredi G, Carelli FN, Rolland SGM, Furlan G, Piquet S, Appert A, Sanchez-Pulido L, Price JL, Alcon P, Lampersberger L, Déclais AC, Ramakrishna NB, Toth R, Macartney T, Alabert C, Ponting CP, Polo SE, Miska EA, Gartner A, Ahringer J, Rouse J.
The histone chaperone SPT2 regulates chromatin structure and function in Metazoa.
Nat Struct Mol Biol. 2024 Mar;31(3) ; PMC7615752

Giacomini G, Piquet S, Chevallier O, Dabin J, Bai SK, Kim B, Siddaway R, Raught B, Coyaud E, Shan CM, Reid RJD, Toda T, Rothstein R, Barra V, Wilhelm T, Hamadat S, Bertin C, Crane A, Dubois F, Forne I, Imhof A, Bandopadhayay P, Beroukhim R, Naim V, Jia S, Hawkins C, Rondinelli B, Polo SE
Aberrant DNA repair reveals a vulnerability in histone H3.3-mutant brain tumors.
Nucleic Acids Res. 2024 Mar 21;52(5) ; PMC10954481

Granados A, Zamperoni M, Rapone R, Moulin M, Boyarchuk E, Bouyioukos C, Del Maestro L, Joliot V, Negroni E, Mohamed M, Piquet S, Bigot A, Le Grand F, Albini S, Ait-Si-Ali S
SETDB1 modulates the TGFβ response in Duchenne muscular dystrophy myotubes.
Sci Adv. 2024 May 3;10(18) ; PMC11062573

Bogard B, Francastel C, Hubé F.
Systematic Identification and Functional Validation of New snoRNAs in Human Muscle Progenitors.
Non-Coding RNA 2021 Sep 13;7(3), 56; PMC8482216

Déborah Bouvier, Juliette Ferrand, Odile Chevallier, Michelle T Paulsen, Mats Ljungman, Sophie E Polo.
Dissecting regulatory pathways for transcription recovery following DNA damage reveals a non-canonical function of the histone chaperone HIRA.
Nat Commun 2021 Jun 22;12(1):3835; PMC8219801

Gabrielle Olley, Madapura M Pradeepa, Graeme R Grimes, Sandra Piquet, Sophie E Polo, David R FitzPatrick, Wendy A Bickmore, Charlene Boumendil.
Cornelia de Lange syndrome-associated mutations cause a DNA damage signalling and repair defect
Nat Commun 2021 May 25;12(1):3127; PMC8149872

Anna Fortuny, Audrey Chansard, Pierre Caron, Odile Chevallier, Olivier Leroy, Olivier Renaud, Sophie E Polo.
Imaging the response to DNA damage in heterochromatin domains reveals core principles of heterochromatin maintenance
Nat Commun 2021 Apr 23;12(1):2428; PMC8065061

Casanova M., Moscatelli M., Chauvière EL., Huret C., Samson J., Liyakat Ali TM., Rosspopoff O. and Rougeulle C.
A primate-specific retroviral enhancer wires the XACT lncRNA into the core pluripotency network in humans.
Nat. Commun. 2019 Dec 11;10(1):5652 ; PMC31827084

Naciri I, Laisné M, Ferry L, Bourmaud M, Gupta N, Di Carlo S, Huna A, Martin N, Peduto L, Bernard D, Kirsh O, Defossez PA.
Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.
Nucleic Acids Res. 2019 Apr 23;47(7):3407-3421; PMC6468300

Kori S, Ferry L, Matano S, Jimenji T, Kodera N, Tsusaka T, Matsumura R, Oda T, Sato M, Dohmae N, Ando T, Shinkai Y, Defossez PA, Arita K.
Structure of the UHRF1 Tandem Tudor Domain Bound to a Methylated Non-histone Protein, LIG1, Reveals Rules for Binding and Regulation.
Structure. 2019 Mar 5;27(3):485-496.e7. DOI: 10.1016/j.str.2018.11.012

Ma X, Warnier M, Raynard C, Ferrand M, Kirsh O, Defossez PA, Martin N, Bernard D.
The nuclear receptor RXRA controls cellular senescence by regulating calcium signaling.
Aging Cell. 2018 Dec;17(6):e12831. PMC6260923

Sandra Piquet, Florent Le Parc, Siau-Kun Bai, Odile Chevallier, Salomé Adam, Sophie E Polo.
The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage
Mol Cell 2018 Dec 6;72(5):888-901.e7 PMC6292839

Marchal C, de Dieuleveult M, Saint-Ruf C, Guinot N, Ferry L, Olalla Saad ST, Lazarini M, Defossez PA, Miotto B.
Depletion of ZBTB38 potentiates the effects of DNA demethylating agents in cancer cells via CDKN1C mRNA up-regulation.
Oncogenesis. 2018 Oct 11;7(10):82. PMC6182000

Miotto B, Marchal C, Adelmant G, Guinot N, Xie P, Marto JA, Zhang L, Defossez PA.
Stabilization of the methyl-CpG binding protein ZBTB38 by the deubiquitinase USP9X limits the occurrence and toxicity of oxidative stress in human cells.
Nucleic Acids Res. 2018 May 18;46(9):4392-4404. PMC5961141

Furlan G., Gutierrez Hernandez N., Huret C., Galupa R., von Bemmel J., Romito A., Heard E., Morey C. and Rougeulle C.
The Ftx noncoding locus controls X chromosome inactivation independently of its RNA products.
Mol. Cell. 2018 May 3; 70:462-472. DOI: 10.1016/j.molcel.2018.03.024

Kilens S., Meistermann D., Moreno D., Chariau C., Gaignerie A., Reignier A., Lelièvre Y., Casanova M., Vallot C., Nedellec S., Flippe L., Firmin J., Song J., Charpentier E., Lammers J., Donnart A., Marec N., Deb  W., Bihouée A., , Le Caignec C., Pecqueur C., Redon R., Barrière P., Bourdon J., Pasque V., Soumillon M., Mikkelsen T.S.,Rougeulle C., Fréour T. and David L.
Parallel derivation of isogenic human primed and naive induced pluripotent stem cells.
Nat. Commun. 2018 Jan 24;9(1):360. PMC5783949

Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y, Defossez PA.
Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation.
Mol Cell. 2017 Aug 17;67(4):550-565.e5. DOI: 10.1016/j.molcel.2017.07.012

Hédouin S, Grillo G, Ivkovic I, Velasco G, Francastel C.
CENP-A chromatin disassembly in stressed and senescent murine cells.
Sci Rep. 2017 Feb 10;7:42520. PMID: 28186195

Vallot C, Patrat C, Collier AJ, Huret C, Casanova M, Liyakat Ali TM, Tosolini M, Frydman N, Heard E, Rugg-Gunn PJ, Rougeulle C.
XACT Noncoding RNA Competes with XIST in the Control of X Chromosome Activity during Human Early Development.
Cell Stem Cell. 2017 Jan 5;20(1):102-111. PMID: 27989768

Roussel-Gervais A, Naciri I, Kirsh O, Kasprzyk L, Velasco G, Grillo G, Dubus P, Defossez PA.
Loss of the methyl-CpG binding protein ZBTB4 alters mitotic checkpoint, increases aneuploidy and promotes tumorigenesis .
Cancer Res . 2016 Nov; pii: canres.1181. PMID: 27815388

Beyer S, Pontis J, Schirwis E, Battisti V, Rudolf A, Le Grand F, Ait-Si-Ali S.
Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation.
Cell Discov. 2016 Oct 18;2:16037. PMID: 27790377

Adam S, Dabin J, Chevallier O, Leroy O, Baldeyron C, Corpet A, Lomonte P, Renaud O, Almouzni G, Polo SE.
Real-Time Tracking of Parental Histones Reveals Their Contribution to Chromatin Integrity Following DNA Damage.
Mol Cell. 2016 Oct 6;64(1):65-78. PMID: 27642047

Cantone I, Bagci H, Dormann D, Dharmalingam G, Nesterova T, Brockdorff N,Rougeulle C, Vallot C, Heard E, Chaligne R, Merkenschlager M, Fisher AG.
Ordered chromatin changes and human X chromosome reactivation by cell fusion-mediated pluripotent reprogramming.
Nat Commun. 2016 Aug 10;7:12354. PMID: 27507283

Battisti V, Pontis J, Boyarchuk E, Fritsch L, Robin P, Ait-Si-Ali S, Joliot V.
Unexpected Distinct Roles of the Related Histone H3 Lysine 9 Methyltransferases G9a and G9a-Like Protein in Myoblasts.
J Mol Biol. 2016 Jun 5;428(11):2329-43. PMID: 27056598

Marsolier J, Perichon M, DeBarry JD, Villoutreix BO, Chluba J, Lopez T,
Garrido C, Zhou XZ, Lu KP, Fritsch L, Ait-Si-Ali S, Mhadhbi M, Medjkane S, Weitzman JB.
Theileria parasites secrete a prolyl isomerase to maintain hostb leukocyte transformation.
Nature. 2015 Apr 16;520(7547):378-82. PMID: 25624101

Vallot C, Ouimette JF, Makhlouf M, Féraud O, Pontis J, Côme J, Martinat C, Bennaceur-Griscelli A, Lalande M, Rougeulle C.
Erosion of X Chromosome Inactivation in Human Pluripotent Cells Initiates with XACT Coating and Depends on a Specific Heterochromatin Landscape.
Cell Stem Cell. 2015 May 7;16(5):533-46. PMID: 25921272

Makhlouf M, Ouimette JF, Oldfield A, Navarro P, Neuillet D, Rougeulle C.
A prominent and conserved role for YY1 in Xist transcriptional activation.
Nat Commun. 2014 Sep 11;5:4878. PMID: 25209548

Miotto B, Chibi M, Xie P, Koundrioukoff S, Moolman-Smook H, Pugh D, Debatisse M, He F, Zhang L, Defossez PA.
The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.
Cell Rep. 2014 Apr 24;7(2):575-87. PMID: 24726359

Vallot C, Huret C, Lesecque Y, Resch A, Oudrhiri N, Bennaceur-Griscelli A, Duret L, Rougeulle C.
XACT, a long noncoding transcript coating the active X chromosome in human pluripotent cells.
Nat Genet. 2013 Mar;45(3):239-41. PMID: 23334669

Sandra Piquet
Centre épigénétique et Destin Cellulaire
CNRS UMR7216 – Université Paris Cité
Lamarck B, 4e étage, pièce 413
35, rue Hélène Brion
75205 Paris Cedex 13

Tel : 33 (0)1 57 27 89 81
Fax : 33 (0)1 57 27 89 11
Email : sandra.piquet@cnrs.fr

Audrey Chansard (back in March 2027)
Centre épigénétique et Destin Cellulaire
CNRS UMR7216 – Université Paris Cité
Lamarck B, 4e étage, pièce 413
35, rue Hélène Brion
75205 Paris Cedex 13

Tel : 33 (0)1 57 27 89 81
Fax : 33 (0)1 57 27 89 11
Email : audrey.chansard@cnrs.fr

Haut-débit Revvity Operetta:
Salle RB70

Confocal UVC laser Zeiss LSM900 Airyscan :
Salle RB71

Plein champs de routine Leica DMI6000, DMRA2, DMIL :
Salle 425

Plein champs spécialisés Zeiss Apotome 2, Leica DMI8 Thunder :
Salle RB74