Confocal Microscopy and UVC Laser
The platform is equipped with a Zeiss LSM900 confocal microscope mounted with quartz optics for the transmission of a 266nm UVC laser. This UVC laser is controlled by a RappOpto module, enabling targeted irradiation of living cells and small organoids.
It is also equipped with a CO2 incubation chamber.
External users interested in using the 266nm UVC laser are invited to contact the platform: epi2imaging@parisepigenetics.com.
© Image by Ralf Vetterle from Pixabay
Technical specifications:
LSM900 "Pink Floyd"
Inverted confocal microscope with heating chamber + CO2
X, Y and Z piloted
Filters and objectives piloted
Camera:
None
Detector:
2 detectors GaAsP decomposed in 2 detectors GaAsP–PMT
Illumination:
Visible light (halogen)
X-cite series 120Q fluorescent lamp (Mercury Halides, 3000h max)
Lasers :
– Diode laser 405nm, 5mW, laser classe 3B
– Diode laser 488nm, 10mW, laser classe 3B
– Diode laser (SHG) 561nm, 10mW, laser classe 3B
– Diode laser 640nm, 5mW, laser classe 3B
Acquisition software:
Zen 3.5 Blue Edition
Objectives:
| Mag. | N.A | Quality | Transmission Contrast |
Imm. | Max resolution at 488nm |
| 10X | 0,3 | EC PLAN NeoFluar | – | Air | 992 nm |
| 40X | 1,3 | Plan Apo (UV)-vis-IR | DIC | Oil | 229 nm |
| 63X | 1,4 | Plan Apo | DIC | Oil | 213 nm |
| 40X | 0,60 | Ultrafluar QUARTZ | – | Glycerol | 496 nm |
| 100X | 1,2 | Ultrafluar QUARTZ | – | Glycerol | 248 nm |
Epifluorescence Filters:
| Name | Colour | Excitation Filter BP | Dichroic Mirror | Suppression Filter BP |
| DAPI | Blue | 335-383 | 395 | 420-470 |
| GFP | Green | 450-490 | 495 | 500-550 |
| Ds-RED | Red | 538-562 | 570 | 570-640 |
UVC Laser
Laser :
266nm pulsed laser, 20kHz, 15mW Class IIIB
Pulse duration: <1ns
Maximum output power: >0.5µJ
Driver module:
UGA-42 Firefly RappOpto Electronic
Irradiation software :
SysCon with “Click&Fire” mode, communicating with Zen Blue
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