High Throughput Microscopy
The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.
Contact the platform for more information.
© Sandra Piquet
Technical specifications :
High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode
Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx
Image size:
2560*2160 px
Pixel size:
6.50 um x 6.50 μm
Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)
Objectives:
Mag. |
N.A |
Quality |
Transmission Contrast |
Imm. |
Pixel size (binning 1) |
Max resolution at 488 nm |
5X |
0,16 |
Perkin WD : 12,1 nm |
– |
Air |
1,3 μm |
2,6 μm |
10X |
0,3 |
Perkin WD : 5,2 nm |
– |
Air |
0,65 μm |
1,3 μm |
20X |
0,4 |
Perkin WD : 8,28 nm |
– |
Air |
0,325 μm |
790 nm |
20X water |
1 |
Perkin WD : 1,7 nm |
– |
Water |
0,325 μm |
660 nm |
40X water |
1,1 |
Perkin WD : 0,62 nm |
– |
Water |
0,1625 μm |
330 nm |
63X water |
1,15 |
Perkin WD : 0,6 nm |
– |
Water |
0,103 μm
|
280 nm |
Filters:
Name |
Colour |
Excitation Filter BP |
Dichroic Mirror |
Suppression Filter BP |
UV |
blue |
380/40 |
425 |
430-500 |
Green |
green |
475/30 |
495 |
500-550 |
Red |
red |
535/30 |
565 |
570-650 |
Far red |
Far red |
630/20 |
650 |
655-760 |
Read more
Professional afterwork – April 22, 2024
Do you know what else exists outside academia?Come to talk about it with our two guests on Monday, April 22, 2024, at 6.15 pm. Where? Epigenetics and Cell Fate Center (EDC)Université Paris Cité - 35 Rue Helene Brion 75013 Paris – Lamarck B building - room 391 ...
Manuel Muñoz seminar
Wednesday, March 6, 2024, at 11:00 am Manuel Muñoz University of Buenos Aires-CONICET, ArgentinaInvited by Sophie Polo “RNA polymerase II abundance is controlled by DNA repair” The seminar will take place in the Institut Jacques Monod seminar room (RB-18B). Buffon...
Jean-Christophe Andrau seminar
Wednesday, February 28, 2024, at 11:00 amJean-Christophe Andrau IGMM, MontpellierInvited by Pierre-Antoine Defossez “Secondary DNA structures contribute to promoter activity as nucleosome exclusion modules” The seminar will take place in the Institut Jacques Monod...
EMBO Workshop “Chromatin dynamics and nuclear organization in genome maintenance”
Sophie Polo is co-organizing the third edition of the EMBO Workshop "Chromatin dynamics and nuclear organization in genome maintenance". © https://meetings.embo.org/event/24-chromatin-dynamics The workshop will take place in Leiden, Netherlands, from June 17 to 20,...