EpiG : Functional epigenomics

The Functional Epigenomics platform’s objectives are to provide equipment and to train staff in its use.
It offers services for epigenome analysis and participates in the development of new techniques according to the evolution of the field.
The Functional Epigenomics platform is open to the academic and industrial scientific community.

Scheme of platform’s expertise

Epigenomic Platform offers support in the design, production and interpretation of epigenomic experiments.

© Laure Ferry

Expertise

The epigenomic platform offers expertise in analysis of epigenetic marks. It evaluates the feasibility of submitted projects and proposes the most suitable method for their completion.

DNA Methylation

  • LUMA (LUminometric Methylation Assay)
  • MeDIP ou hMeDIP (Methylated or hydroxyMethylated Immunoprecipitation Assay)
  • Bisulfite pyrosequencing

Histones Marks 

  • Cut & Run
  • ChIP

Submit a project

You can contact us for any questions or to discuss your project together.

You must fill in the project request form and send it to the following address: parisepigenetics.pf@gmail.com 

Developments

The Functional Epigenomic platform is involved in the development of new techniques for epigenome analysis:

  • Cut & Run automatisation
  • Cut & Tag
  • Enzymatic Methyl-seq ( EM-seq)
  • Nanopore
 

Epigenetic analyses available

Services for DNA methylation and histone marks analyses

To know more

Equipment

Equipment available on the Epigenomic platform

To know more
Members

Scientific manager: Pierre-Antoine Defossez

Technical manager: Laure Ferry 

Steering committee:

Publications
  • Planques A, Pierre K, Ferry L, Grunau C, Gazave E, Vervoort M. DNA methylation atlas and machinery in the developing and regenerating annelid Platynereis dumerilii. BMC Biology, Vol 19, Iss 1, Pp 1-26, 2021. DOI: 10.1186/s12915-021-01074-5
  • Marchal C, de Dieuleveult M, Saint-Ruf C, Guinot N, Ferry L, Olalla Saad ST, Lazarini M, Defossez PA, Miotto B. Depletion of ZBTB38 potentiates the effects of DNA demethylating agents in cancer cells via CDKN1C mRNA up-regulation. Oncogenesis; Oct 2018, Vol. 7 Issue 10, p1-18, 18p DOI: 10.1038/s41389-018-0092-0
  • Velasco G, Grillo G, Touleimat N, Ferry L, Ivkovic I, Ribierre F, Deleuze JF, Chantalat S, Picard C, Francastel C. Comparative methylome analysis of ICF patients identifies heterochromatin loci that require ZBTB24, CDCA7 and HELLS for their methylated state. Human Molecular Genetics, 2018 Jul 15;27(14):2409-2424. DOI: 10.1093/hmg/ddy130.
  • Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y, Defossez PA. Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation. Molecular Cell. August 17, 2017, Vol. 67 Issue 4, p550. DOI: 10.1016/j.molcel.2017.07.012
  • Kebir O, Chaumette B, Rivollier F, Miozzo F, Lemieux Perreault LP, Barhdadi  A, Provost S, Plaze M, Bourgin J, the ICAAR team, Gaillard  R, Mezger V, Dubé  M-P , Krebs  M-O. Methylomic changes during conversion to psychosis.  Molecular Psychiatry (2017) 22, 512-518. DOI: 10.1038/mp.2016.53.
Contact

You can contact us at: parisepigenetics.pf@gmail.com

Functional Epigenomic Platform

35 rue Hélène Brion

Bâtiment Lamarck B, RB77-81

75013 PARIS

+33(0)1 57 27 89 26

 

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