Epigenetic analyses available

Depending on the question of interest, the Epigenomics platform offers different techniques for the analysis of DNA methylation and histone marks.

Scheme of the techniques proposed on the Epigenomic platform.
The Epigenomic platform offers techniques for the analysis of DNA methylation and histone marks.

© Laure Ferry

DNA Methylation analyses

LUMA (LUminometric Methylation Assay)

  • Method for global DNA methylation analysis.
  • Quantification of the number of restriction enzyme cuts, based on methylation sensitivity.
  • Rapid & quantitative determination of methylation level.
  • No information about the location or repartition of DNA methylation.

MeDIP (Methylated DNA ImmunoPrécipitation)

  • Enrichment of fragmented genomic DNA using an antibody specific for 5mC
  • Low CpG dinucleotide density may not be accurately analyzed by MeDIP.
  • 5mC antibodies are capable of discrimination between 5-hmC and 5-mC.

Bisulfite Pyrosequencing

  • Analysis & quantification of the methylation degree of several CpG positions in close proximity.
  • Bisulfite-based methods cannot differentiate 5-mC and 5-hmC.

Histones Marks analyses

CUT & RUN (Cleavage Under Targets & Release Using Nuclease)

  • Powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell
  • CUT&RUN provides a rapid, robust with low cell number assay
  • Free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a more efficient method for identifying target genes.

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Congratulations to Dr. Silvia Comunian!

Congratulations to Dr. Silvia Comunian!

On September 25, 2026, Silvia successfully defended her thesis: “Epigenetic control of nuclear mechanics and migration via H3K9 methylation at Lamina-Associated Domains in lung cancer cells”Silvia Comunian  and the jury members: Yegor Vassetzky, Nicolas Borghi,...

Welcome to Sara

Welcome to Sara

Sara is joining the team for a master’s internship. As a student in the Interdisciplinary Master’s in Life Sciences program at the Ecole Normale Superieure, Sara will contribute to a research project aimed at identifying vulnerabilities in DNA repair in high-grade...