GENIE : Genomic engineering in epigenetics
The “Genome ENgeneering In Epigenetics” (GENIE) platform focuses on the various applications of CRISPR/Cas technologies in the field of epigenetics.
CRISPR/Cas-based applications for epigenetic studies
Presentation
GENIE assists researchers in the planning of experiments and the assembly of reagents for the generation of custom experimental systems. We provide expertise and tools for various genome and epigenome engineering applications.
Our goal is to facilitate the choice of genome editing, epigenome and other tools based on CRISPR/Cas technologies. GENIE is open to all members of the UMR7216 Epigenetics and Cell Fate unit and to a certain extent to members of the transverse programs of the Labex “Who am I?”. The acceptance of external projects is possible after discussion with the steering committee and depends on the resources available.
Expertise
Our expertise lies in the following areas:
- Genome editing: knock-out, knock-in, and point mutation introduction
- Modulation of gene expression and modification of the epigenome
- Use of CRISPR/Cas technologies for imaging or for identification of protein composition of the locus of interest using proximity tagging techniques
- CRISPR/Cas targeting of RNA for degradation, imaging, or editing
Services
- Access to the bank of molecular tools for genome engineering, epigenomes and RNA modifications.
- Protocols, advice, and technical support for genome and epigenome engineering and other applications based on CRISPR/Cas technologies.
- Implementations of novel CRISPR/Cas-based approaches adapted to the needs of the unit.
- Training for new users in CRISPR-based technologies in a form of a course on genome and epigenome editing.
In collaboration with other platforms of the unit, GENIE assists in determining the optimal guide sequences and/or recombination template and in designing the optimal engineering strategy for each project, with an emphasis on easy downstream screening. We also help to choose the best way to deliver components for (epi)gene editing into cells. Besides the classical transfection reagents or nucleofection available in the EDC teams, full support can be provided for lentiviral delivery systems in collaboration with Vectorology platform.
Members
Resources
Bank of molecular tools for genome and epigenome engineering
To get access to GENIE’s bank of vectors, contact us
Contact
GENIE
Lamarck B building, room 411
35 rue Hélène Brion, 75013 Paris
e-mail: genie@parisepigenetics.com
Steering committee
Joined steering committee for GENIE and Vectorology platforms:
-
- Slimane Ait-Si-Ali
- Pierre-Antoine Defossez
- Aurélie de Thonel
- Claire Rougeulle
- Christophe Huret (Vectorology platform manager)
- Ekaterina Boyarchuk (GENIE platform manager)
Read more
Welcome to Léa, new engineer in the team!
Léa joins the lab as a research assistant. She holds a Master's degree in Molecular and Cellular Biology from Sorbonne University. She will contribute to investigate DNA methylation maintenance mechanisms in response to UV damage in mammalian cells. Léa Girard À lire...
Well done, Dr Mori!
Margherita successfully defended her PhD on DNA methylation maintenance in response to UV damage. Brava! Margherita and her thesis jury. From left to right: Sophie Polo, Sandra Duharcourt (on screen), Déborah Bourc'his, Margherita Mori, Nataliya Petryk, Jean Molinier,...
Congratulations Dr Giacomini!
Giulia defended her PhD on the impact of high grade glioma-associated histone H3 mutations on genome integrity. Congratulations to her on a fantastic thesis defense! Giulia Giacomini and her thesis jury. From left to right: Beatrice Rondinelli, Keith Caldecott,...
Congratulations to Dr. Maeva Zamperoni!
On September 15, 2025, Maeva successfully defended her thesis: “Role of the lysine methyltransferase SETDB1 in TGFβ-induced fibrosis in Duchenne Muscular Dystrophy (DMD).”Maeva and the jury members: Pascal Maire, Nicolas Reynoird, Sestina Falcone, Frédérique...