High Throughput Microscopy
The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.
Contact the platform for more information.
© Sandra Piquet
Technical specifications :
High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode
Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx
Image size:
2560*2160 px
Pixel size:
6.50 um x 6.50 μm
Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)
Objectives:
|
Mag. |
N.A |
Quality |
Transmission Contrast |
Imm. |
Pixel size (binning 1) |
Max resolution at 488 nm |
|
5X |
0,16 |
Perkin WD : 12,1 nm |
– |
Air |
1,3 μm |
2,6 μm |
|
10X |
0,3 |
Perkin WD : 5,2 nm |
– |
Air |
0,65 μm |
1,3 μm |
|
20X |
0,4 |
Perkin WD : 8,28 nm |
– |
Air |
0,325 μm |
790 nm |
|
20X water |
1 |
Perkin WD : 1,7 nm |
– |
Water |
0,325 μm |
660 nm |
|
40X water |
1,1 |
Perkin WD : 0,62 nm |
– |
Water |
0,1625 μm |
330 nm |
|
63X water |
1,15 |
Perkin WD : 0,6 nm |
– |
Water |
0,103 μm
|
280 nm |
Filters:
|
Name |
Colour |
Excitation Filter BP |
Dichroic Mirror |
Suppression Filter BP |
|
UV |
blue |
380/40 |
425 |
430-500 |
|
Green |
green |
475/30 |
495 |
500-550 |
|
Red |
red |
535/30 |
565 |
570-650 |
|
Far red |
Far red |
630/20 |
650 |
655-760 |
Read more
Congratulations to the Dr. Kevin Daupin
Congratulations to Dr. Kevin Daupin who defended his thesis this Wednesday, December 4, 2024 on « Integrity of Neuroepithelium & the Neural Niche in Rubinstein-Taybi Syndrome Derived Neural Models : A perspective on the implication of HSF2 pathways dysregulations...
Welcome to Megan
Today, we welcome a new member to the team: Megan, a M1 student from the EPHE where she's doing the Integrative Master for Global Health and Ecology, Integrative Physiopathology course.Megan will be working with Souhila on lysine methylation signalling by SMYD2.Read...
New review: Unleashing XIST from X-chromosome inactivation
The discovery that long noncoding RNAs (lncRNAs) are the most abundant gene class in the human genome has sparked significant research, particularly on Xist, a key RNA involved in X-chromosome inactivation. Recent studies have expanded our understanding of Xist's...
Congratulations to Emmanuelle !
Last week, Emmanuelle Ceddaha, one of our PhD student, defended her pharmacy thesis. It was a great presentation which not only earned her the congratulations of the jury, but also the distinction of ‘very good’ (and the unofficial distinction of ‘excellent’) for her...