High Throughput Microscopy
The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.
Contact the platform for more information.
© Sandra Piquet
Technical specifications :
High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode
Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx
Image size:
2560*2160 px
Pixel size:
6.50 um x 6.50 μm
Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)
Objectives:
|
Mag. |
N.A |
Quality |
Transmission Contrast |
Imm. |
Pixel size (binning 1) |
Max resolution at 488 nm |
|
5X |
0,16 |
Perkin WD : 12,1 nm |
– |
Air |
1,3 μm |
2,6 μm |
|
10X |
0,3 |
Perkin WD : 5,2 nm |
– |
Air |
0,65 μm |
1,3 μm |
|
20X |
0,4 |
Perkin WD : 8,28 nm |
– |
Air |
0,325 μm |
790 nm |
|
20X water |
1 |
Perkin WD : 1,7 nm |
– |
Water |
0,325 μm |
660 nm |
|
40X water |
1,1 |
Perkin WD : 0,62 nm |
– |
Water |
0,1625 μm |
330 nm |
|
63X water |
1,15 |
Perkin WD : 0,6 nm |
– |
Water |
0,103 μm
|
280 nm |
Filters:
|
Name |
Colour |
Excitation Filter BP |
Dichroic Mirror |
Suppression Filter BP |
|
UV |
blue |
380/40 |
425 |
430-500 |
|
Green |
green |
475/30 |
495 |
500-550 |
|
Red |
red |
535/30 |
565 |
570-650 |
|
Far red |
Far red |
630/20 |
650 |
655-760 |
Read more
Seminar Jan Padeken –October 14, 2026
Dr. Jan Padeken Institute of Molecular Biology, Mainz, Germany "UV-Damage-induced Epigenetic Memory in Organismal Resilience and Aging." The seminar will take place in the Institut Jacques Monod seminar room (RB-18B).Bâtiment Buffon, 15 rue Hélène Brion, Paris 13th....
Seminar Philipp Voigt –October 7, 2026
Dr. Philipp Voigt Babraham Institute, Cambridge, UK "Decoding bivalency: Mechanisms of poising at developmental gene promoters." The seminar will take place in the Institut Jacques Monod seminar room (RB-18B).Bâtiment Buffon, 15 rue Hélène Brion, Paris 13th. On...
Congratulations to Dr. Silvia Comunian!
On September 25, 2026, Silvia successfully defended her thesis: “Epigenetic control of nuclear mechanics and migration via H3K9 methylation at Lamina-Associated Domains in lung cancer cells”Silvia Comunian and the jury members: Yegor Vassetzky, Nicolas Borghi,...
Welcome to Sara
Sara is joining the team for a master’s internship. As a student in the Interdisciplinary Master’s in Life Sciences program at the Ecole Normale Superieure, Sara will contribute to a research project aimed at identifying vulnerabilities in DNA repair in high-grade...