Epigenetic analyses available

Depending on the question of interest, the Epigenomics platform offers different techniques for the analysis of DNA methylation and histone marks.

Scheme of the techniques proposed on the Epigenomic platform.
The Epigenomic platform offers techniques for the analysis of DNA methylation and histone marks.

© Laure Ferry

DNA Methylation analyses

LUMA (LUminometric Methylation Assay)

  • Method for global DNA methylation analysis.
  • Quantification of the number of restriction enzyme cuts, based on methylation sensitivity.
  • Rapid & quantitative determination of methylation level.
  • No information about the location or repartition of DNA methylation.

MeDIP (Methylated DNA ImmunoPrécipitation)

  • Enrichment of fragmented genomic DNA using an antibody specific for 5mC
  • Low CpG dinucleotide density may not be accurately analyzed by MeDIP.
  • 5mC antibodies are capable of discrimination between 5-hmC and 5-mC.

Bisulfite Pyrosequencing

  • Analysis & quantification of the methylation degree of several CpG positions in close proximity.
  • Bisulfite-based methods cannot differentiate 5-mC and 5-hmC.

Histones Marks analyses

CUT & RUN (Cleavage Under Targets & Release Using Nuclease)

  • Powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell
  • CUT&RUN provides a rapid, robust with low cell number assay
  • Free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a more efficient method for identifying target genes.

Read more

Thomas Graf seminar

Thomas Graf seminar

Monday, December 4, 2023, at 10:00 am Thomas Graf CRG, BarcelonaInvited by Pierre-Antoine Defossez “Mechanisms of cell fate conversions” The seminar will take place on Zoom only:https://u-paris.zoom.us/j/86114422072?pwd=VzJ0c2pxcHl4OWppekx3MldlM2Y4QT09 Contact:...

Yoichi Shinkai seminar

Yoichi Shinkai seminar

Wednesday, November 22, 2023, at 11:00 am Yoichi Shinkai RIKEN Saitama, JapanInvited by Pierre-Antoine Defossez “Lysine methyltransferase EHMT1/GLP (Ehmt1) heterozygous KO mice as a Kleefstra syndrome model: challenging the onset mechanism and developing therapeutic...

We celebrated Science in October

We celebrated Science in October

Every October, we celebrate science through the "Fête de la Science", a popular public event that celebrates science, technology and innovation. It is open to all and helps promote the sharing of knowledge. This year, our Centre was involved in two visits. Our...