DNA methylation in stem cells

One of our research axes is focused on understanding how DNA methylation patterns are established, maintained, and interpreted during embryonic development. To address these questions we use mouse embryonic stem (ES) cells as our model, and we apply a wide array of tools: cell biology, genomics, proteomics, bioinformatics… This has led us, for example, to decipher important mechanisms of epigenetic memory (Ferry et al, Mol Cell 2017, review in Petryk et al NAR 2020). More recently, we carried out genome-wide CRISPR screens to identify factors linking epigenetics and cellular state (Gupta, Yakhou et al., NSMB 2023).

A CRISPR screen identifies new repressors of the totipotent state in mouse embryonic stem cells

In mammals, only the zygote and blastomeres of the early embryo are fully totipotent. This totipotency is reflected in vitro by the expression of markers such as Zscan4. We performed a genome-wide CRISPR KO screen in mouse embryonic stem cells, looking for mutants that reactivate the expression of totipotency markers, like for example the knock-out of Spop.

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Manuel Muñoz seminar

Manuel Muñoz seminar

Wednesday, March 6, 2024, at 11:00 am Manuel Muñoz University of Buenos Aires-CONICET, ArgentinaInvited by Sophie Polo “RNA polymerase II abundance is controlled by DNA repair” The seminar will take place in the Institut Jacques Monod seminar room (RB-18B). Buffon...

Jean-Christophe Andrau seminar

Jean-Christophe Andrau seminar

Wednesday, February 28, 2024, at 11:00 amJean-Christophe Andrau IGMM, MontpellierInvited by Pierre-Antoine Defossez “Secondary DNA structures contribute to promoter activity as nucleosome exclusion modules” The seminar will take place in the Institut Jacques Monod...