High Throughput Microscopy

The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.

Contact the platform for more information.

Operetta CLS, PerkinElmer ®

© Sandra Piquet

Technical specifications :

High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode

Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx

Image size:
2560*2160 px

Pixel size:
6.50 um x 6.50 μm

Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)

Objectives:

Mag.

N.A

Quality

Transmission

Contrast

Imm.

Pixel size

(binning 1)

Max resolution at 488 nm

5X

0,16

Perkin WD : 12,1 nm

–

Air

1,3 μm

2,6 μm

10X

0,3

Perkin WD : 5,2 nm

–

Air

0,65 μm

1,3 μm

20X

0,4

Perkin WD : 8,28 nm

–

Air

0,325 μm

790 nm

20X water

1

Perkin WD : 1,7 nm

–

Water

0,325 μm

660 nm

40X water

1,1

Perkin WD : 0,62 nm

–

Water

0,1625 μm

330 nm

63X water

1,15

Perkin WD : 0,6 nm

–

Water

0,103 μm

 

 

280 nm

Filters:

Name

Colour

Excitation Filter BP

Dichroic Mirror

Suppression Filter BP

UV

blue

380/40

425

430-500

Green

green

475/30

495

500-550

Red

red

535/30

565

570-650

Far red

Far red

630/20

650

655-760

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