High Throughput Microscopy
The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.
Contact the platform for more information.
© Sandra Piquet
Technical specifications :
High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode
Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx
Image size:
2560*2160 px
Pixel size:
6.50 um x 6.50 μm
Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)
Objectives:
|
Mag. |
N.A |
Quality |
Transmission Contrast |
Imm. |
Pixel size (binning 1) |
Max resolution at 488 nm |
|
5X |
0,16 |
Perkin WD : 12,1 nm |
– |
Air |
1,3 μm |
2,6 μm |
|
10X |
0,3 |
Perkin WD : 5,2 nm |
– |
Air |
0,65 μm |
1,3 μm |
|
20X |
0,4 |
Perkin WD : 8,28 nm |
– |
Air |
0,325 μm |
790 nm |
|
20X water |
1 |
Perkin WD : 1,7 nm |
– |
Water |
0,325 μm |
660 nm |
|
40X water |
1,1 |
Perkin WD : 0,62 nm |
– |
Water |
0,1625 μm |
330 nm |
|
63X water |
1,15 |
Perkin WD : 0,6 nm |
– |
Water |
0,103 μm
|
280 nm |
Filters:
|
Name |
Colour |
Excitation Filter BP |
Dichroic Mirror |
Suppression Filter BP |
|
UV |
blue |
380/40 |
425 |
430-500 |
|
Green |
green |
475/30 |
495 |
500-550 |
|
Red |
red |
535/30 |
565 |
570-650 |
|
Far red |
Far red |
630/20 |
650 |
655-760 |
Read more
Welcome to Orlane
Today, we welcome a new member to the team: Orlane. She's a student from the first year of the Brevet de Technicien Supérieur Biotechnologies en Recherche et Production (Two-year technical degree - Biotechnologies in Research and Production).Orlane will be working...
4th year PhD fellowships for Anaëlle
Congratulations to Anaëlle Azogui for obtaining a 4th year PhD fellowships from the "Fondation pour la recherche sur le cancer" (Fondation ARC). Read more
Congratulations to the Defossez team for their article published in Nature Communication
Congratulations to the Defossez team for their article “DNA methylation protects cancer cells against senescence” by Chen, Yamaguchi, et al., which has been accepted in Nature Communication. Read it here: doi:doi: https://doi.org/10.1101/2024.08.23.609297...
Welcome to Delphine, new post-doc in the team!
Delphine joins the lab as a post-doctoral fellow. She completed her PhD at the Centre de Recherche en Cancérologie de Lyon (CRCL) under the supervision of Virginie Petrilli. Her doctoral work focused on the pro-inflammatory protein NLRP3, for which she highlighted a...