Epigenetic analyses available
Depending on the question of interest, the Epigenomics platform offers different techniques for the analysis of DNA methylation and histone marks.
Scheme of the techniques proposed on the Epigenomic platform.
The Epigenomic platform offers techniques for the analysis of DNA methylation and histone marks.
© Laure Ferry
DNA Methylation analyses
LUMA (LUminometric Methylation Assay)
- Method for global DNA methylation analysis.
- Quantification of the number of restriction enzyme cuts, based on methylation sensitivity.
- Rapid & quantitative determination of methylation level.
- No information about the location or repartition of DNA methylation.
MeDIP (Methylated DNA ImmunoPrécipitation)
-
Enrichment of fragmented genomic DNA using an antibody specific for 5mC
-
Low CpG dinucleotide density may not be accurately analyzed by MeDIP.
-
5mC antibodies are capable of discrimination between 5-hmC and 5-mC.
Bisulfite Pyrosequencing
-
Analysis & quantification of the methylation degree of several CpG positions in close proximity.
-
Bisulfite-based methods cannot differentiate 5-mC and 5-hmC.
Histones Marks analyses
CUT & RUN (Cleavage Under Targets & Release Using Nuclease)
-
Powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell
-
CUT&RUN provides a rapid, robust with low cell number assay
-
Free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a more efficient method for identifying target genes.
Read more
New review: Genetic diseases of epigenetic machinery
The development of sequencing technologies and their increased accessibility in clinical services and genetic laboratories have considerably accelerated the identification of genetic variants associated with rare diseases. Among these, Mendelian disorders of the...
Congratulations to Guillaume!
Congratulations to Dr Guillaume Velasco on obtaining his Habilitation à Diriger des Recherches (HDR) this Wednesday, December 18th. A well-deserved accomplishment!Jury members: Giséle Bonne, Jean-Charles Cadoret, Fabienne Malagnac and Guillermo OrsiCongratulations,...
Congratulations to Dr. Carole Chaput
Congratulations to Dr. Carole Chaput who defended her thesis this Friday, December 6, 2024 on « Therapeutic functionalization of a rare neurodevelopmental and monogenic disease model based on the contribution of the heat shock factor 2 stress pathway »À lire aussi
Congratulations to Dr. Kevin Daupin
Congratulations to Dr. Kevin Daupin who defended his thesis this Wednesday, December 4, 2024 on « Integrity of Neuroepithelium & the Neural Niche in Rubinstein-Taybi Syndrome Derived Neural Models : A perspective on the implication of HSF2 pathways dysregulations...