Epigenetic analyses available
Depending on the question of interest, the Epigenomics platform offers different techniques for the analysis of DNA methylation and histone marks.
Scheme of the techniques proposed on the Epigenomic platform.
The Epigenomic platform offers techniques for the analysis of DNA methylation and histone marks.
© Laure Ferry
DNA Methylation analyses
LUMA (LUminometric Methylation Assay)
- Method for global DNA methylation analysis.
- Quantification of the number of restriction enzyme cuts, based on methylation sensitivity.
- Rapid & quantitative determination of methylation level.
- No information about the location or repartition of DNA methylation.
MeDIP (Methylated DNA ImmunoPrécipitation)
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Enrichment of fragmented genomic DNA using an antibody specific for 5mC
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Low CpG dinucleotide density may not be accurately analyzed by MeDIP.
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5mC antibodies are capable of discrimination between 5-hmC and 5-mC.
Bisulfite Pyrosequencing
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Analysis & quantification of the methylation degree of several CpG positions in close proximity.
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Bisulfite-based methods cannot differentiate 5-mC and 5-hmC.
Histones Marks analyses
CUT & RUN (Cleavage Under Targets & Release Using Nuclease)
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Powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell
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CUT&RUN provides a rapid, robust with low cell number assay
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Free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a more efficient method for identifying target genes.
Read more
Welcome to Léa, new engineer in the team!
Léa joins the lab as a research assistant. She holds a Master's degree in Molecular and Cellular Biology from Sorbonne University. She will contribute to investigate DNA methylation maintenance mechanisms in response to UV damage in mammalian cells. Léa Girard À lire...
Well done, Dr Mori!
Margherita successfully defended her PhD on DNA methylation maintenance in response to UV damage. Brava! Margherita and her thesis jury. From left to right: Sophie Polo, Sandra Duharcourt (on screen), Déborah Bourc'his, Margherita Mori, Nataliya Petryk, Jean Molinier,...
Congratulations Dr Giacomini!
Giulia defended her PhD on the impact of high grade glioma-associated histone H3 mutations on genome integrity. Congratulations to her on a fantastic thesis defense! Giulia Giacomini and her thesis jury. From left to right: Beatrice Rondinelli, Keith Caldecott,...
Congratulations to Dr. Maeva Zamperoni!
On September 15, 2025, Maeva successfully defended her thesis: “Role of the lysine methyltransferase SETDB1 in TGFβ-induced fibrosis in Duchenne Muscular Dystrophy (DMD).”Maeva and the jury members: Pascal Maire, Nicolas Reynoird, Sestina Falcone, Frédérique...