High Throughput Microscopy

The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.

Contact the platform for more information.

Operetta CLS, PerkinElmer ®

Technical specifications :

High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode

Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx

Image size:
2560*2160 px

Pixel size:
6.50 um x 6.50 μm

Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)

Objectives:

Mag.	N.A	Quality	Transmission Contrast	Imm.	Pixel size (binning 1)	Max resolution at 488 nm
5X	0,16	Perkin WD : 12,1 nm	–	Air	1,3 μm	2,6 μm
10X	0,3	Perkin WD : 5,2 nm	–	Air	0,65 μm	1,3 μm
20X	0,4	Perkin WD : 8,28 nm	–	Air	0,325 μm	790 nm
20X water	1	Perkin WD : 1,7 nm	–	Water	0,325 μm	660 nm
40X water	1,1	Perkin WD : 0,62 nm	–	Water	0,1625 μm	330 nm
63X water	1,15	Perkin WD : 0,6 nm	–	Water	0,103 μm	280 nm

Filters:

Name	Colour	Excitation Filter BP	Dichroic Mirror	Suppression Filter BP
UV	blue	380/40	425	430-500
Green	green	475/30	495	500-550
Red	red	535/30	565	570-650
Far red	Far red	630/20	650	655-760

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