Epigenetic analyses available
Depending on the question of interest, the Epigenomics platform offers different techniques for the analysis of DNA methylation and histone marks.
Scheme of the techniques proposed on the Epigenomic platform.
The Epigenomic platform offers techniques for the analysis of DNA methylation and histone marks.
© Laure Ferry
DNA Methylation analyses
LUMA (LUminometric Methylation Assay)
- Method for global DNA methylation analysis.
- Quantification of the number of restriction enzyme cuts, based on methylation sensitivity.
- Rapid & quantitative determination of methylation level.
- No information about the location or repartition of DNA methylation.
MeDIP (Methylated DNA ImmunoPrécipitation)
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Enrichment of fragmented genomic DNA using an antibody specific for 5mC
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Low CpG dinucleotide density may not be accurately analyzed by MeDIP.
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5mC antibodies are capable of discrimination between 5-hmC and 5-mC.
Bisulfite Pyrosequencing
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Analysis & quantification of the methylation degree of several CpG positions in close proximity.
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Bisulfite-based methods cannot differentiate 5-mC and 5-hmC.
Histones Marks analyses
CUT & RUN (Cleavage Under Targets & Release Using Nuclease)
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Powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell
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CUT&RUN provides a rapid, robust with low cell number assay
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Free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a more efficient method for identifying target genes.
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Postdoctoral position in epigenetics and cancer
Applications are invited for a 3-year postdoctoral position in the laboratory of Dr. Sophie Polo. The position is available from January 2025 (open until filled). The proposed postdoctoral work aims at targeting the DNA repair defect that we identified in histone H3...
EDC15: Fifteen Years of Epigenetics and Cell Fate
The Epigenetics and Cell Fate Center celebrates its fifteen years of existence on October 8, 2024. Come, and join us for this special day! This conference will look back on 15 years of scientific advances in the fields of epigenetics and cellular differentiation in...
2024 Young Scientists Day
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Call for new Research Group Leaders
The Epigenetics and Cell Fate Centre is seeking to recruit two talented junior and/or senior group leaders.Download the call (.pdf) Download the application form (.doc) The Epigenetics and Cell Fate Centre is a leading institute exploring cell identity and...